LOCUS pYFP-F 8788 bp DNA circular 12-MAR-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|656351010| COMMENT VNTDBDATE|656351010| COMMENT LSOWNER| COMMENT VNTNAME|pYFP-F for website| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS complement(7723..8583) /vntifkey="4" /label=AmpR CDS complement(2051..2701) /vntifkey="4" /label=G+\CmR misc_marker 4801..5966 /vntifkey="22" /label=pE194\ORI /note="HhaI or CfoI fragment of pE194 known to replicate" rep_origin complement(6941..7570) /vntifkey="33" /label=pBR322\ori misc_feature 3207..4536 /vntifkey="21" /label=counter\selection misc_feature 7..516 /vntifkey="21" /label=TN\up misc_feature 1280..1806 /vntifkey="21" /label=TN\down CDS 554..1273 /vntifkey="4" /label=EYFP misc_feature 526..553 /vntifkey="21" /label=TIR BASE COUNT 2671 a 1719 c 1605 g 2793 t ORIGIN 1 gaattctctc tagacacata gatggcgtcg cggcgcgccg gagatataca tatgagtaaa 61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat 121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc 181 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact 241 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt 301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac 361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc 421 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac 481 aactataact cacacaatgt atacatcatg gcaggctaag ctaggtgatt aactttataa 541 ggaggaaaaa catatggttt caaaaggtga agaattattt acaggtgttg ttccaatttt 601 ggttgaatta gatggtgatg ttaatggtca taaattttca gtttcaggtg aaggtgaagg 661 tgatgcaact tatggtaaat taacattaaa atttatttgt acaacaggta aattaccagt 721 tccatggcca acattagtta caacatttgg ttatggttta caatgttttg cacgttatcc 781 agatcacatg aaacaacatg atttttttaa atcagcaatg cctgaaggtt atgttcaaga 841 acgtacaatt ttttttaaag atgatggcaa ctataaaaca cgtgctgaag ttaaatttga 901 aggtgataca ttagttaatc gtattgaatt aaaaggtatt gattttaaag aagatggtaa 961 tattttaggt cataaattag aatataatta taactcacat aatgtttata tcatggcaga 1021 taaacaaaaa aatggtatta aagttaattt taaaattcgt cataatattg aagatggttc 1081 agttcaatta gcagatcatt atcaacaaaa tacacctatt ggtgatggtc cagttttatt 1141 accagataat cattatttat catatcaatc agcattatca aaagatccaa atgaaaaacg 1201 tgatcacatg gttttattag aatttgttac agcagcaggt attacattag gtatggatga 1261 attatataaa taacctagcg ccataccaca gatgttccag ataaatattg gaagctatat 1321 acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac taaaaatcag 1381 tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccgttac ttatgagcaa 1441 gtattgtcta tttttaatag ttatctatta tttaacggga ggaaataatt ctatgagtcg 1501 cttttgtaaa tttggaaagt tacacgttac taaagggaat gtagataaat tattaggtat 1561 actactgaca gcttccaagg agctaaagag gtccctagcg cctacgggga atttgtatcg 1621 ataaggaata gatttaaaaa tttcgctgtt attttgtaca ataaggataa atttgaatgg 1681 taccataaac gaccgtttat ggtaccattt cattttcctg ctttttctaa atgtttttta 1741 agtaaatcaa gtaccaaaat ccgttccttt ttcatagttc ctatatagtt atacgcgtct 1801 agagacgtcg acctgcaggc atgcaagctc tgatttcact ttttgcattc tacaaactgc 1861 ataactcata tgtaaatcgc tcctttttag gtggcacaaa tgtgaggcat tttcgctctt 1921 tccggcgagg ctagttaccc ttaagttatt ggtatgactg gttttaagcg caaaaaaagt 1981 tgctttttcg tacctattaa tgtatcgtta gaaaaccgac tgtaaaaagt acagtcggca 2041 ttatctcata ttataaaagc cagtcattag gcctatctga caattcctga atagagttca 2101 taaacaatcc tgcatgataa ccatcacaaa cagaatgatg tacctgtaaa gatagcggta 2161 aatatattga attaccttta ttaatgaatt ttcctgctgt aataatgggt agaaggtaat 2221 tactattatt attgatattt aagttaaacc cagtaaatga agtccatgga ataatagaaa 2281 gagaaaaagc attttcaggt ataggtgttt tgggaaacaa tttccccgaa ccattatatt 2341 tctctacatc agaaaggtat aaatcataaa actctttgaa gtcattcttt acaggagtcc 2401 aaataccaga gaatgtttta gatacaccat caaaaattgt ataaagtggc tctaacttat 2461 cccaataacc taactctccg tcgctattgt aaccagttct aaaagctgta tttgagttta 2521 tcacccttgt cactaagaaa ataaatgcag ggtaaaattt atatccttct tgttttatgt 2581 ttcggtataa aacactaata tcaatttctg tggttatact aaaagtcgtt tgttggttca 2641 aataatgatt aaatatctct tttctcttcc aattgtctaa atcaatttta ttaaagttca 2701 tttgatatgc ctcctaaatt tttatctaaa gtgaatttag gaggcttact tgtctgcttt 2761 cttcattaga atcaatcctt ttttaaaagt caatattact gtaacataaa tatatatttt 2821 aaaaatatcc cactttatcc aattttcgtt tgttgaacta atgggtgctt tagttgaaga 2881 ataaagacca cattaaaaaa tgtggtcttt tgtgtttttt taaaggattt gagcgtagcg 2941 aaaaatcctt ttctttctta tcttgataat aagggtaact attgccggga tagactgtaa 3001 cattctcacg cataaaatcc cctttcattt tctaatgtaa atctattacc ttattattaa 3061 ttcaattcgc tcataattaa tcctttttct tattacgcaa aatggcccga tttaagcaca 3121 ccctttattc cgttaatgcg ccatgacagc catgataatt actaatacta ggagaagtta 3181 ataaatacgg gtgatctaat gattcaatga ttcaaaccct tgtgaacttc tttagaacaa 3241 aagaggttcg taacaagatt ttcttcacac tagcaatgtt agtaattttt aaaataggga 3301 cttatatacc agctccagga gtaaatcctg cagcttttga taatccccaa ggttctcaag 3361 gtgccactga gttattaaat acttttggtg gcggagcctt gaaacgattt tctatttttg 3421 caatgggtat tgtaccctac atcactgcat caatcgtaat gcaattatta caaatggata 3481 ttgtccctaa attctcagaa tgggcaaaac aaggtgaagt aggtagaaga aagttaaata 3541 acgttactcg ttatttagca atttctttag catttatcca atctataggt atggcattcc 3601 aatttaataa ttatctcaaa ggtgcgctga ttatcaatca gtcaattatg agttatttat 3661 taatagcact agttttgaca gcaggaactg ctttcttaat atggcttggt gatcaaatca 3721 ctcagttcgg tgttggtaat ggtatttcta ttatcatatt cccatcaagc ttattttaat 3781 tatactctat caatgataga gtgtcaatat tttttttagt ttttcatgaa ctcgatcgag 3841 gggatccaaa taaaaaacta gtttgacaaa taactctatc aatgatagag tgtcaacaaa 3901 aaggaggaat taatgatgtc tagattagat aaaagtaaag tgattaacag cgcattagag 3961 ctgcttaatg aggtcggaat cgaaggttta acaacccgta aactcgccca gaagctaggt 4021 gtagagcagc ctacattgta ttggcatgta aaaaataagc gggctttgct cgacgcctta 4081 gccattgaga tgttagatag gcaccatact cacttttgcc ctttagaagg ggaaagctgg 4141 caagattttt tacgtaataa cgctaaaagt tttagatgtg ctttactaag tcatcgcgat 4201 ggagcaaaag tacatttagg tacacggcct acagaaaaac agtatgaaac tctcgaaaat 4261 caattagcct ttttatgcca acaaggtttt tcactagaga atgcattata tgcactcagc 4321 gctgtggggc attttacttt aggttgcgta ttggaagatc aagagcatca agtcgctaaa 4381 gaagaaaggg aaacacctac tactgatagt atgccgccat tattacgaca agctatcgaa 4441 ttatttgatc accaaggtgc agagccagcc ttcttattcg gccttgaatt gatcatatgc 4501 ggattagaaa aacaacttaa atgtgaaagt gggtcttaaa agcagcataa cctttttccg 4561 tgatggtaac ttcacggtaa ccaagataac aaagaataca agaaaatatt tacaaaaaat 4621 caatttaaca attccttaaa acatgcagga attgacgatt taaacaatat tagctttgaa 4681 caattcttat ctcttttcaa tagctataaa ttatttaata agtaagttaa gggatgcata 4741 aactgcatcc cttaacttgt ttttcgtgtg cctatttttt gtgaatcgat tatgtctttt 4801 gcgcagtcgg cttaaaccag ttttcgctgg tgcgaaaaaa gagtgtcttg tgacacctaa 4861 attcaaaatc tatcggtcag atttataccg atttgatttt atatattctt gaataacata 4921 cgccgagtta tcacataaaa gcgggaacca atcatcaaat ttaaacttca ttgcataatc 4981 cattaaactc ttaaattcta cgattccttg ttcatcaata aactcaatca tttctttaat 5041 taatttatat ctatctgttg ttgttttctt taataattca tcaacatcta caccgccata 5101 aactatcata tcttcttttt gatatttaaa tttattagga tcgtccatgt gaagcatata 5161 tctcacaaga cctttcacac ttcctgcaat ctgcggaata gtcgcattca attcttctgt 5221 aattattttt atctgttcat aagatttatt accctcatac atcactagaa tatgataatg 5281 ctcttttttc atcctatctt ctgtatcagt atccctatca tgtaatggag acactacaaa 5341 ttgaatgtgt aactctttta aatactctaa ccactcggct tttgctgatt ctggatataa 5401 aacaaatgtc caattacgtc ctcttgaatt tttcttgttt tcagtttctt ttattacatt 5461 ttcgctcatg atataataac ggtgctaata catttaacaa aatttagtca tagataggca 5521 gcatgccagt gctgtctatc tttttttgtt taaaatgcac cgtattcctc ctttgcatat 5581 ttttttatta gaataccggt tgcatctgat ttgctaatat tatatttttc tttgattcta 5641 tttaatatct cattttcttc tgttgtaagt cttaaagtaa cagcaacttt tttctcttct 5701 tttctatcta caaccatcac tgtacctccc aacatctgtt tttttcactt taacataaaa 5761 aacaaccttt taacattaaa aacccaatat ttatttattt gtttggacaa tggacaatgg 5821 acacctaggg gggaggtcgt agtacccccc tatgttttct cccctaaata accccaaaaa 5881 tctaagaaaa aaagacctca aaaaggtctt taattaacat ctcaaatttc gcatttattc 5941 caatttcctt tttgcgtgtg atgcgctgcg tccattaaaa atcctagagc tttgcaaccg 6001 aaagttaata gctgtcgcta ctactttcgc ttacgctcta agtatatttt aaggactgtc 6061 acacgcaaaa agttttctcg gcataaaagt acctctacat ctctaaatcg tctgtacgct 6121 gtttctcacg ctttctatcg accttctgga cattatcctg tacaacatcc ataaactgtc 6181 ccacacgctc gaatttggaa tcattaaaga atttctcttt aagcctatta aaccctttct 6241 caaacccagg gaaattcgcc ctcgcagcac gatataaagt cactgtacta tcttgaaatt 6301 tctctgatac attcaactgc tcattcaaac tatcattctc tcgctttaat ttattaacct 6361 ctttactttt ttcgtgatac ccctctttcc atgtattcac tacttctttc aaactctctc 6421 tacgtttttt taattcttga ttttctgtgt aatagtctgt gctcttaata ttttcgtaat 6481 catcaacaat ccgttcctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 6541 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 6601 gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta 6661 gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt 6721 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg 6781 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 6841 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 6901 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 6961 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 7021 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 7081 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 7141 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 7201 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 7261 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 7321 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 7381 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 7441 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 7501 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 7561 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 7621 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 7681 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 7741 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 7801 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 7861 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 7921 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 7981 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc 8041 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 8101 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 8161 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 8221 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 8281 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac 8341 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 8401 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 8461 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 8521 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 8581 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 8641 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 8701 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 8761 gcgtatcacg aggccctttc gtcttcaa //