LOCUS pTN 8034 bp DNA circular 24-JUL-2012 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|632494831| COMMENT VNTDBDATE|632495525| COMMENT LSOWNER| COMMENT VNTNAME|pTN for NARSA| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS complement(6969..7829) /vntifkey="4" /label=AmpR CDS complement(1297..1947) /vntifkey="4" /label=G+\CmR rep_origin 4047..5212 /vntifkey="33" /label=pE194ts /note="HhaI or CfoI fragment of pE194 known to replicate" rep_origin complement(6187..6816) /vntifkey="33" /label=pBR322\ori misc_feature 2453..3782 /vntifkey="21" /label=counter\selection misc_signal 7..516 /vntifkey="87" /label=TN\up misc_marker 526..1052 /vntifkey="22" /label=TN\down BASE COUNT 2392 a 1634 c 1475 g 2533 t ORIGIN 1 gaattctctc tagacacata gatggcgtcg cggcgcgccg gagatataca tatgagtaaa 61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat 121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc 181 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact 241 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt 301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac 361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc 421 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac 481 aactataact cacacaatgt atacatcatg gcaggctaag ctagcgccat accacagatg 541 ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc aatcgagaat 601 atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc aaagtaaaca 661 atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat ctattattta 721 acgggaggaa ataattctat gagtcgcttt tgtaaatttg gaaagttaca cgttactaaa 781 gggaatgtag ataaattatt aggtatacta ctgacagctt ccaaggagct aaagaggtcc 841 ctagcgccta cggggaattt gtatcgataa ggaatagatt taaaaatttc gctgttattt 901 tgtacaataa ggataaattt gaatggtacc ataaacgacc gtttatggta ccatttcatt 961 ttcctgcttt ttctaaatgt tttttaagta aatcaagtac caaaatccgt tcctttttca 1021 tagttcctat atagttatac gcgtctagag acgtcgacct gcaggcatgc aagctctgat 1081 ttcacttttt gcattctaca aactgcataa ctcatatgta aatcgctcct ttttaggtgg 1141 cacaaatgtg aggcattttc gctctttccg gcgaggctag ttacccttaa gttattggta 1201 tgactggttt taagcgcaaa aaaagttgct ttttcgtacc tattaatgta tcgttagaaa 1261 accgactgta aaaagtacag tcggcattat ctcatattat aaaagccagt cattaggcct 1321 atctgacaat tcctgaatag agttcataaa caatcctgca tgataaccat cacaaacaga 1381 atgatgtacc tgtaaagata gcggtaaata tattgaatta cctttattaa tgaattttcc 1441 tgctgtaata atgggtagaa ggtaattact attattattg atatttaagt taaacccagt 1501 aaatgaagtc catggaataa tagaaagaga aaaagcattt tcaggtatag gtgttttggg 1561 aaacaatttc cccgaaccat tatatttctc tacatcagaa aggtataaat cataaaactc 1621 tttgaagtca ttctttacag gagtccaaat accagagaat gttttagata caccatcaaa 1681 aattgtataa agtggctcta acttatccca ataacctaac tctccgtcgc tattgtaacc 1741 agttctaaaa gctgtatttg agtttatcac ccttgtcact aagaaaataa atgcagggta 1801 aaatttatat ccttcttgtt ttatgtttcg gtataaaaca ctaatatcaa tttctgtggt 1861 tatactaaaa gtcgtttgtt ggttcaaata atgattaaat atctcttttc tcttccaatt 1921 gtctaaatca attttattaa agttcatttg atatgcctcc taaattttta tctaaagtga 1981 atttaggagg cttacttgtc tgctttcttc attagaatca atcctttttt aaaagtcaat 2041 attactgtaa cataaatata tattttaaaa atatcccact ttatccaatt ttcgtttgtt 2101 gaactaatgg gtgctttagt tgaagaataa agaccacatt aaaaaatgtg gtcttttgtg 2161 tttttttaaa ggatttgagc gtagcgaaaa atccttttct ttcttatctt gataataagg 2221 gtaactattg ccgggataga ctgtaacatt ctcacgcata aaatcccctt tcattttcta 2281 atgtaaatct attaccttat tattaattca attcgctcat aattaatcct ttttcttatt 2341 acgcaaaatg gcccgattta agcacaccct ttattccgtt aatgcgccat gacagccatg 2401 ataattacta atactaggag aagttaataa atacgggtga tctaatgatt caatgattca 2461 aacccttgtg aacttcttta gaacaaaaga ggttcgtaac aagattttct tcacactagc 2521 aatgttagta atttttaaaa tagggactta tataccagct ccaggagtaa atcctgcagc 2581 ttttgataat ccccaaggtt ctcaaggtgc cactgagtta ttaaatactt ttggtggcgg 2641 agccttgaaa cgattttcta tttttgcaat gggtattgta ccctacatca ctgcatcaat 2701 cgtaatgcaa ttattacaaa tggatattgt ccctaaattc tcagaatggg caaaacaagg 2761 tgaagtaggt agaagaaagt taaataacgt tactcgttat ttagcaattt ctttagcatt 2821 tatccaatct ataggtatgg cattccaatt taataattat ctcaaaggtg cgctgattat 2881 caatcagtca attatgagtt atttattaat agcactagtt ttgacagcag gaactgcttt 2941 cttaatatgg cttggtgatc aaatcactca gttcggtgtt ggtaatggta tttctattat 3001 catattccca tcaagcttat tttaattata ctctatcaat gatagagtgt caatattttt 3061 tttagttttt catgaactcg atcgagggga tccaaataaa aaactagttt gacaaataac 3121 tctatcaatg atagagtgtc aacaaaaagg aggaattaat gatgtctaga ttagataaaa 3181 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa 3241 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa 3301 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact 3361 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta 3421 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag 3481 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac 3541 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg 3601 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc 3661 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct 3721 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt 3781 cttaaaagca gcataacctt tttccgtgat ggtaacttca cggtaaccaa gataacaaag 3841 aatacaagaa aatatttaca aaaaatcaat ttaacaattc cttaaaacat gcaggaattg 3901 acgatttaaa caatattagc tttgaacaat tcttatctct tttcaatagc tataaattat 3961 ttaataagta agttaaggga tgcataaact gcatccctta acttgttttt cgtgtgccta 4021 ttttttgtga atcgattatg tcttttgcgc agtcggctta aaccagtttt cgctggtgcg 4081 aaaaaagagt gtcttgtgac acctaaattc aaaatctatc ggtcagattt ataccgattt 4141 gattttatat attcttgaat aacatacgcc gagttatcac ataaaagcgg gaaccaatca 4201 tcaaatttaa acttcattgc ataatccatt aaactcttaa attctacgat tccttgttca 4261 tcaataaact caatcatttc tttaattaat ttatatctat ctgttgttgt tttctttaat 4321 aattcatcaa catctacacc gccataaact atcatatctt ctttttgata tttaaattta 4381 ttaggatcgt ccatgtgaag catatatctc acaagacctt tcacacttcc tgcaatctgc 4441 ggaatagtcg cattcaattc ttctgtaatt atttttatct gttcataaga tttattaccc 4501 tcatacatca ctagaatatg ataatgctct tttttcatcc tatcttctgt atcagtatcc 4561 ctatcatgta atggagacac tacaaattga atgtgtaact cttttaaata ctctaaccac 4621 tcggcttttg ctgattctgg atataaaaca aatgtccaat tacgtcctct tgaatttttc 4681 ttgttttcag tttcttttat tacattttcg ctcatgatat aataacggtg ctaatacatt 4741 taacaaaatt tagtcataga taggcagcat gccagtgctg tctatctttt tttgtttaaa 4801 atgcaccgta ttcctccttt gcatattttt ttattagaat accggttgca tctgatttgc 4861 taatattata tttttctttg attctattta atatctcatt ttcttctgtt gtaagtctta 4921 aagtaacagc aacttttttc tcttcttttc tatctacaac catcactgta cctcccaaca 4981 tctgtttttt tcactttaac ataaaaaaca accttttaac attaaaaacc caatatttat 5041 ttatttgttt ggacaatgga caatggacac ctagggggga ggtcgtagta cccccctatg 5101 ttttctcccc taaataaccc caaaaatcta agaaaaaaag acctcaaaaa ggtctttaat 5161 taacatctca aatttcgcat ttattccaat ttcctttttg cgtgtgatgc gctgcgtcca 5221 ttaaaaatcc tagagctttg caaccgaaag ttaatagctg tcgctactac tttcgcttac 5281 gctctaagta tattttaagg actgtcacac gcaaaaagtt ttctcggcat aaaagtacct 5341 ctacatctct aaatcgtctg tacgctgttt ctcacgcttt ctatcgacct tctggacatt 5401 atcctgtaca acatccataa actgtcccac acgctcgaat ttggaatcat taaagaattt 5461 ctctttaagc ctattaaacc ctttctcaaa cccagggaaa ttcgccctcg cagcacgata 5521 taaagtcact gtactatctt gaaatttctc tgatacattc aactgctcat tcaaactatc 5581 attctctcgc tttaatttat taacctcttt acttttttcg tgatacccct ctttccatgt 5641 attcactact tctttcaaac tctctctacg tttttttaat tcttgatttt ctgtgtaata 5701 gtctgtgctc ttaatatttt cgtaatcatc aacaatccgt tcctgcctcg cgcgtttcgg 5761 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 5821 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 5881 gggcgcagcc atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg 5941 gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc 6001 gtaaggagaa aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc 6061 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 6121 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 6181 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 6241 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 6301 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 6361 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 6421 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 6481 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 6541 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 6601 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 6661 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 6721 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 6781 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 6841 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 6901 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 6961 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 7021 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 7081 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 7141 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 7201 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 7261 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg 7321 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 7381 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 7441 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 7501 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 7561 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta 7621 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 7681 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 7741 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 7801 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 7861 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 7921 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 7981 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct tcaa //