LOCUS pTET 10265 bp DNA circular 12-MAR-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|656344996| COMMENT VNTDBDATE|656344996| COMMENT LSOWNER| COMMENT VNTNAME|pTET for website| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS complement(9200..10060) /vntifkey="4" /label=AmpR CDS complement(3528..4178) /vntifkey="4" /label=G+\CmR misc_marker 6278..7443 /vntifkey="22" /label=pE194\ORI /note="HhaI or CfoI fragment of pE194 known to replicate" rep_origin complement(8418..9047) /vntifkey="33" /label=pBR322\ori misc_feature 4684..6013 /vntifkey="21" /label=counter\selection misc_feature 7..516 /vntifkey="21" /label=TN\up misc_feature 2757..3283 /vntifkey="21" /label=TN\down misc_feature 829..2748 /vntifkey="21" /label=tetA(M) BASE COUNT 3155 a 1973 c 1918 g 3219 t ORIGIN 1 gaattctctc tagacacata gatggcgtcg cggcgcgccg gagatataca tatgagtaaa 61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat 121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc 181 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact 241 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt 301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac 361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc 421 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac 481 aactataact cacacaatgt atacatcatg gcaggctaag ctaggcaaat atgctcttac 541 gtgctattat ttaagtgact atttaaaagg agttaataaa tatgcggcaa ggtattctta 601 aataaactgt caatttgata gcgggaacaa ataattagat gtcctttttt aggagggctt 661 agttttttgt acccagttta agaatacctt tatcatgtga ttctaaagta tccagagaat 721 atctgtatgc tttgtatacc tatggttatg cataaaaatc ccagtgataa aagtatttat 781 cactgggatt tttatgccct tttgggtttt tgaatggagg aaaatcacat gaaaattatt 841 aatattggag ttttagctca tgttgatgca ggaaaaacta ccttaacaga aagcttatta 901 tataacagtg gagcgattac agaattagga agcgtggaca aaggtacaac gaggacggat 961 aatacgcttt tagaacgtca gagaggaatt acaattcaga caggaataac ctcttttcag 1021 tgggaaaata cgaaggtgaa catcatagac acgccaggac atatggattt cttagcagaa 1081 gtatatcgtt cattatcagt tttagatggg gcaattctac tgatttctgc aaaagatttc 1141 gtacaagcac aaactcgtat attatttcat gcacttagga aaatggggat tcccacaatc 1201 ttttttatca ataagattga ccaaaatgga attgatttat caacggttta tcaggatatt 1261 aaagagaaac tttctgccga aattgtaatc aaacagaagg tagaactgta tcctaatatg 1321 tgtgtgacga actttaccga atctgaacaa tgggatacgg taatagaggg aaacgatgac 1381 cttttagaga aatatatgtc cggtaaatca ttagaagcat tggaactcga acaagaggaa 1441 agcataagat ttcagaattg ttctctgttc cctctttatc atggaagtgc aaaaagtaat 1501 atagggattg ataaccttat agaagttatt actaataaat tttattcatc aacacatcga 1561 ggtccgtctg aactttgcgg aaatgttttc aaaattgaat atacaaaaaa aagacaacgt 1621 cttgcatata tacgccttta tagtggagta ctacatttac gagattcggt tagagtatca 1681 gaaaaagaaa aaataaaagt tacagaaatg tatacttcaa taaatggtga attatgtaag 1741 attgatagag cttattctgg agaaattgtt attttgcaaa atgagttttt gaagttaaat 1801 agtgttcttg gagatacaaa actattgcca cagagaaaaa agattgaaaa tccgcaccct 1861 ctactacaaa caactgttga accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc 1921 cttttggaaa tctcagatag tgatccgctt ctacgatatt acgtggattc tacgacacat 1981 gaaattatac tttctttctt agggaaagta caaatggaag tgattagtgc actgttgcaa 2041 gaaaagtatc atgtggagat agaactaaaa gagcctacag tcatttatat ggagagaccg 2101 ttaaaaaatg cagaatatac cattcacatc gaagtgccgc caaatccttt ctgggcttcc 2161 attggtttat ctgtatcacc gcttccgttg ggaagtggaa tgcagtatga gagctcggtt 2221 tctcttggat acttaaatca atcatttcaa aatgcagtta tggaagggat acgctatggt 2281 tgcgaacaag gattatatgg ttggaatgtg acggattgta aaatctgttt taagtacggt 2341 ttatactata gccctgttag tactccagca gattttcgga tgcttactcc tattgtactg 2401 gagcaagcct ttagaaaagc tggaacagaa ttgttagagc catatcttag ttttaaagtt 2461 tatgcaccac aggaatatct ttcacgggca tataacgatg ctcccaaata ttgtgcaaat 2521 atcgtaaata ctcaactgaa aaataatgag gtcattatta ttggagaaat tcctgctcga 2581 tgtattcaag attatcgcaa tgatttaact ttttttacaa atgggcttag tgtttgttta 2641 gcagagctaa aaggatatca ggttaccact ggcgaacctg tttgccagac ccgtcgtcta 2701 aatagtcgga tagataaagt aagatatatg ttcaataaaa taacttagtg cctagcgcca 2761 taccacagat gttccagata aatattggaa gctatatacg tactttgttt caaaatgggt 2821 caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa tgaaacacgc 2881 caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt ttaatagtta 2941 tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt ggaaagttac 3001 acgttactaa agggaatgta gataaattat taggtatact actgacagct tccaaggagc 3061 taaagaggtc cctagcgcct acggggaatt tgtatcgata aggaatagat ttaaaaattt 3121 cgctgttatt ttgtacaata aggataaatt tgaatggtac cataaacgac cgtttatggt 3181 accatttcat tttcctgctt tttctaaatg ttttttaagt aaatcaagta ccaaaatccg 3241 ttcctttttc atagttccta tatagttata cgcgtctaga gacgtcgacc tgcaggcatg 3301 caagctctga tttcactttt tgcattctac aaactgcata actcatatgt aaatcgctcc 3361 tttttaggtg gcacaaatgt gaggcatttt cgctctttcc ggcgaggcta gttaccctta 3421 agttattggt atgactggtt ttaagcgcaa aaaaagttgc tttttcgtac ctattaatgt 3481 atcgttagaa aaccgactgt aaaaagtaca gtcggcatta tctcatatta taaaagccag 3541 tcattaggcc tatctgacaa ttcctgaata gagttcataa acaatcctgc atgataacca 3601 tcacaaacag aatgatgtac ctgtaaagat agcggtaaat atattgaatt acctttatta 3661 atgaattttc ctgctgtaat aatgggtaga aggtaattac tattattatt gatatttaag 3721 ttaaacccag taaatgaagt ccatggaata atagaaagag aaaaagcatt ttcaggtata 3781 ggtgttttgg gaaacaattt ccccgaacca ttatatttct ctacatcaga aaggtataaa 3841 tcataaaact ctttgaagtc attctttaca ggagtccaaa taccagagaa tgttttagat 3901 acaccatcaa aaattgtata aagtggctct aacttatccc aataacctaa ctctccgtcg 3961 ctattgtaac cagttctaaa agctgtattt gagtttatca cccttgtcac taagaaaata 4021 aatgcagggt aaaatttata tccttcttgt tttatgtttc ggtataaaac actaatatca 4081 atttctgtgg ttatactaaa agtcgtttgt tggttcaaat aatgattaaa tatctctttt 4141 ctcttccaat tgtctaaatc aattttatta aagttcattt gatatgcctc ctaaattttt 4201 atctaaagtg aatttaggag gcttacttgt ctgctttctt cattagaatc aatccttttt 4261 taaaagtcaa tattactgta acataaatat atattttaaa aatatcccac tttatccaat 4321 tttcgtttgt tgaactaatg ggtgctttag ttgaagaata aagaccacat taaaaaatgt 4381 ggtcttttgt gtttttttaa aggatttgag cgtagcgaaa aatccttttc tttcttatct 4441 tgataataag ggtaactatt gccgggatag actgtaacat tctcacgcat aaaatcccct 4501 ttcattttct aatgtaaatc tattacctta ttattaattc aattcgctca taattaatcc 4561 tttttcttat tacgcaaaat ggcccgattt aagcacaccc tttattccgt taatgcgcca 4621 tgacagccat gataattact aatactagga gaagttaata aatacgggtg atctaatgat 4681 tcaatgattc aaacccttgt gaacttcttt agaacaaaag aggttcgtaa caagattttc 4741 ttcacactag caatgttagt aatttttaaa atagggactt atataccagc tccaggagta 4801 aatcctgcag cttttgataa tccccaaggt tctcaaggtg ccactgagtt attaaatact 4861 tttggtggcg gagccttgaa acgattttct atttttgcaa tgggtattgt accctacatc 4921 actgcatcaa tcgtaatgca attattacaa atggatattg tccctaaatt ctcagaatgg 4981 gcaaaacaag gtgaagtagg tagaagaaag ttaaataacg ttactcgtta tttagcaatt 5041 tctttagcat ttatccaatc tataggtatg gcattccaat ttaataatta tctcaaaggt 5101 gcgctgatta tcaatcagtc aattatgagt tatttattaa tagcactagt tttgacagca 5161 ggaactgctt tcttaatatg gcttggtgat caaatcactc agttcggtgt tggtaatggt 5221 atttctatta tcatattccc atcaagctta ttttaattat actctatcaa tgatagagtg 5281 tcaatatttt ttttagtttt tcatgaactc gatcgagggg atccaaataa aaaactagtt 5341 tgacaaataa ctctatcaat gatagagtgt caacaaaaag gaggaattaa tgatgtctag 5401 attagataaa agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga 5461 aggtttaaca acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg 5521 gcatgtaaaa aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca 5581 ccatactcac ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc 5641 taaaagtttt agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac 5701 acggcctaca gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca 5761 aggtttttca ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg 5821 ttgcgtattg gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac 5881 tgatagtatg ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga 5941 gccagccttc ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg 6001 tgaaagtggg tcttaaaagc agcataacct ttttccgtga tggtaacttc acggtaacca 6061 agataacaaa gaatacaaga aaatatttac aaaaaatcaa tttaacaatt ccttaaaaca 6121 tgcaggaatt gacgatttaa acaatattag ctttgaacaa ttcttatctc ttttcaatag 6181 ctataaatta tttaataagt aagttaaggg atgcataaac tgcatccctt aacttgtttt 6241 tcgtgtgcct attttttgtg aatcgattat gtcttttgcg cagtcggctt aaaccagttt 6301 tcgctggtgc gaaaaaagag tgtcttgtga cacctaaatt caaaatctat cggtcagatt 6361 tataccgatt tgattttata tattcttgaa taacatacgc cgagttatca cataaaagcg 6421 ggaaccaatc atcaaattta aacttcattg cataatccat taaactctta aattctacga 6481 ttccttgttc atcaataaac tcaatcattt ctttaattaa tttatatcta tctgttgttg 6541 ttttctttaa taattcatca acatctacac cgccataaac tatcatatct tctttttgat 6601 atttaaattt attaggatcg tccatgtgaa gcatatatct cacaagacct ttcacacttc 6661 ctgcaatctg cggaatagtc gcattcaatt cttctgtaat tatttttatc tgttcataag 6721 atttattacc ctcatacatc actagaatat gataatgctc ttttttcatc ctatcttctg 6781 tatcagtatc cctatcatgt aatggagaca ctacaaattg aatgtgtaac tcttttaaat 6841 actctaacca ctcggctttt gctgattctg gatataaaac aaatgtccaa ttacgtcctc 6901 ttgaattttt cttgttttca gtttctttta ttacattttc gctcatgata taataacggt 6961 gctaatacat ttaacaaaat ttagtcatag ataggcagca tgccagtgct gtctatcttt 7021 ttttgtttaa aatgcaccgt attcctcctt tgcatatttt tttattagaa taccggttgc 7081 atctgatttg ctaatattat atttttcttt gattctattt aatatctcat tttcttctgt 7141 tgtaagtctt aaagtaacag caactttttt ctcttctttt ctatctacaa ccatcactgt 7201 acctcccaac atctgttttt ttcactttaa cataaaaaac aaccttttaa cattaaaaac 7261 ccaatattta tttatttgtt tggacaatgg acaatggaca cctagggggg aggtcgtagt 7321 acccccctat gttttctccc ctaaataacc ccaaaaatct aagaaaaaaa gacctcaaaa 7381 aggtctttaa ttaacatctc aaatttcgca tttattccaa tttccttttt gcgtgtgatg 7441 cgctgcgtcc attaaaaatc ctagagcttt gcaaccgaaa gttaatagct gtcgctacta 7501 ctttcgctta cgctctaagt atattttaag gactgtcaca cgcaaaaagt tttctcggca 7561 taaaagtacc tctacatctc taaatcgtct gtacgctgtt tctcacgctt tctatcgacc 7621 ttctggacat tatcctgtac aacatccata aactgtccca cacgctcgaa tttggaatca 7681 ttaaagaatt tctctttaag cctattaaac cctttctcaa acccagggaa attcgccctc 7741 gcagcacgat ataaagtcac tgtactatct tgaaatttct ctgatacatt caactgctca 7801 ttcaaactat cattctctcg ctttaattta ttaacctctt tacttttttc gtgatacccc 7861 tctttccatg tattcactac ttctttcaaa ctctctctac gtttttttaa ttcttgattt 7921 tctgtgtaat agtctgtgct cttaatattt tcgtaatcat caacaatccg ttcctgcctc 7981 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca 8041 gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt 8101 ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc 8161 ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac 8221 cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg 8281 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 8341 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 8401 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 8461 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 8521 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 8581 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 8641 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 8701 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 8761 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 8821 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 8881 ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 8941 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 9001 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 9061 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 9121 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 9181 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 9241 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 9301 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 9361 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 9421 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 9481 cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt 9541 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 9601 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 9661 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 9721 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 9781 gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata 9841 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 9901 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 9961 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 10021 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 10081 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 10141 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag 10201 aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc 10261 ttcaa //