LOCUS pSPC 9156 bp DNA circular 12-MAR-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|632587779| COMMENT VNTDBDATE|656344876| COMMENT LSOWNER| COMMENT VNTNAME|pSPC for NARSA| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS complement(8091..8951) /vntifkey="4" /label=AmpR CDS complement(2419..3069) /vntifkey="4" /label=G+\CmR rep_origin 5169..6334 /vntifkey="33" /label=pE194\ORI /note="HhaI or CfoI fragment of pE194 known to replicate" rep_origin complement(7309..7938) /vntifkey="33" /label=pBR322\ori misc_feature 3575..4904 /vntifkey="21" /label=counter\selection misc_feature 7..516 /vntifkey="21" /label=TN\up misc_feature 1648..2174 /vntifkey="21" /label=TN\down repeat_region 517..1641 /vntifkey="34" /label=aad9 BASE COUNT 2807 a 1796 c 1700 g 2853 t ORIGIN 1 gaattctctc tagacacata gatggcgtcg cggcgcgccg gagatataca tatgagtaaa 61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat 121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc 181 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact 241 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt 301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac 361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc 421 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac 481 aactataact cacacaatgt atacatcatg gcaggctaag ctaggatcga atcccttcgt 541 gagcatcaaa taacagcaaa tgtagtctca aaacccaaga aaagttctcg ttcggaggaa 601 atccttattg aaaccttaaa aagaagagta atggaattag aaaaagaaaa taaaaaatta 661 cagaaccaaa ttcaaaaatt atatggagat ctgtataata aagaataatt attaatctgt 721 agacaaattg tgaaaggatg tacttaaacg ctaacggtca gctttattga acagtaattt 781 aagtatatgt ccaatctagg gtaagtaaat tgagtatcaa tataaacttt atatgaacat 841 aatcaacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa tcaagcgatt 901 caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt atatctattt 961 ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct agtcgtcgtg 1021 aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat gactatatca 1081 ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat aaataggagt 1141 gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga gtggctcagg 1201 ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt ggctattgtt 1261 ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc aagtatactt 1321 gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc agaactaatt 1381 gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat gtggcaaaca 1441 gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat acctctttta 1501 cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg agagtgtgat 1561 gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat gaaaaattct 1621 atagaaactt ctctcaatta gcctagcgcc ataccacaga tgttccagat aaatattgga 1681 agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa ctgtttacta 1741 aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt accgttactt 1801 atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg aaataattct 1861 atgagtcgct tttgtaaatt tggaaagtta cacgttacta aagggaatgt agataaatta 1921 ttaggtatac tactgacagc ttccaaggag ctaaagaggt ccctagcgcc tacggggaat 1981 ttgtatcgat aaggaataga tttaaaaatt tcgctgttat tttgtacaat aaggataaat 2041 ttgaatggta ccataaacga ccgtttatgg taccatttca ttttcctgct ttttctaaat 2101 gttttttaag taaatcaagt accaaaatcc gttccttttt catagttcct atatagttat 2161 acgcgtctag agacgtcgac ctgcaggcat gcaagctctg atttcacttt ttgcattcta 2221 caaactgcat aactcatatg taaatcgctc ctttttaggt ggcacaaatg tgaggcattt 2281 tcgctctttc cggcgaggct agttaccctt aagttattgg tatgactggt tttaagcgca 2341 aaaaaagttg ctttttcgta cctattaatg tatcgttaga aaaccgactg taaaaagtac 2401 agtcggcatt atctcatatt ataaaagcca gtcattaggc ctatctgaca attcctgaat 2461 agagttcata aacaatcctg catgataacc atcacaaaca gaatgatgta cctgtaaaga 2521 tagcggtaaa tatattgaat tacctttatt aatgaatttt cctgctgtaa taatgggtag 2581 aaggtaatta ctattattat tgatatttaa gttaaaccca gtaaatgaag tccatggaat 2641 aatagaaaga gaaaaagcat tttcaggtat aggtgttttg ggaaacaatt tccccgaacc 2701 attatatttc tctacatcag aaaggtataa atcataaaac tctttgaagt cattctttac 2761 aggagtccaa ataccagaga atgttttaga tacaccatca aaaattgtat aaagtggctc 2821 taacttatcc caataaccta actctccgtc gctattgtaa ccagttctaa aagctgtatt 2881 tgagtttatc acccttgtca ctaagaaaat aaatgcaggg taaaatttat atccttcttg 2941 ttttatgttt cggtataaaa cactaatatc aatttctgtg gttatactaa aagtcgtttg 3001 ttggttcaaa taatgattaa atatctcttt tctcttccaa ttgtctaaat caattttatt 3061 aaagttcatt tgatatgcct cctaaatttt tatctaaagt gaatttagga ggcttacttg 3121 tctgctttct tcattagaat caatcctttt ttaaaagtca atattactgt aacataaata 3181 tatattttaa aaatatccca ctttatccaa ttttcgtttg ttgaactaat gggtgcttta 3241 gttgaagaat aaagaccaca ttaaaaaatg tggtcttttg tgttttttta aaggatttga 3301 gcgtagcgaa aaatcctttt ctttcttatc ttgataataa gggtaactat tgccgggata 3361 gactgtaaca ttctcacgca taaaatcccc tttcattttc taatgtaaat ctattacctt 3421 attattaatt caattcgctc ataattaatc ctttttctta ttacgcaaaa tggcccgatt 3481 taagcacacc ctttattccg ttaatgcgcc atgacagcca tgataattac taatactagg 3541 agaagttaat aaatacgggt gatctaatga ttcaatgatt caaacccttg tgaacttctt 3601 tagaacaaaa gaggttcgta acaagatttt cttcacacta gcaatgttag taatttttaa 3661 aatagggact tatataccag ctccaggagt aaatcctgca gcttttgata atccccaagg 3721 ttctcaaggt gccactgagt tattaaatac ttttggtggc ggagccttga aacgattttc 3781 tatttttgca atgggtattg taccctacat cactgcatca atcgtaatgc aattattaca 3841 aatggatatt gtccctaaat tctcagaatg ggcaaaacaa ggtgaagtag gtagaagaaa 3901 gttaaataac gttactcgtt atttagcaat ttctttagca tttatccaat ctataggtat 3961 ggcattccaa tttaataatt atctcaaagg tgcgctgatt atcaatcagt caattatgag 4021 ttatttatta atagcactag ttttgacagc aggaactgct ttcttaatat ggcttggtga 4081 tcaaatcact cagttcggtg ttggtaatgg tatttctatt atcatattcc catcaagctt 4141 attttaatta tactctatca atgatagagt gtcaatattt tttttagttt ttcatgaact 4201 cgatcgaggg gatccaaata aaaaactagt ttgacaaata actctatcaa tgatagagtg 4261 tcaacaaaaa ggaggaatta atgatgtcta gattagataa aagtaaagtg attaacagcg 4321 cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa ctcgcccaga 4381 agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg gctttgctcg 4441 acgccttagc cattgagatg ttagataggc accatactca cttttgccct ttagaagggg 4501 aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct ttactaagtc 4561 atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag tatgaaactc 4621 tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat gcattatatg 4681 cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa gagcatcaag 4741 tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta ttacgacaag 4801 ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc cttgaattga 4861 tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtcttaaaag cagcataacc 4921 tttttccgtg atggtaactt cacggtaacc aagataacaa agaatacaag aaaatattta 4981 caaaaaatca atttaacaat tccttaaaac atgcaggaat tgacgattta aacaatatta 5041 gctttgaaca attcttatct cttttcaata gctataaatt atttaataag taagttaagg 5101 gatgcataaa ctgcatccct taacttgttt ttcgtgtgcc tattttttgt gaatcgatta 5161 tgtcttttgc gcagtcggct taaaccagtt ttcgctggtg cgaaaaaaga gtgtcttgtg 5221 acacctaaat tcaaaatcta tcggtcagat ttataccgat ttgattttat atattcttga 5281 ataacatacg ccgagttatc acataaaagc gggaaccaat catcaaattt aaacttcatt 5341 gcataatcca ttaaactctt aaattctacg attccttgtt catcaataaa ctcaatcatt 5401 tctttaatta atttatatct atctgttgtt gttttcttta ataattcatc aacatctaca 5461 ccgccataaa ctatcatatc ttctttttga tatttaaatt tattaggatc gtccatgtga 5521 agcatatatc tcacaagacc tttcacactt cctgcaatct gcggaatagt cgcattcaat 5581 tcttctgtaa ttatttttat ctgttcataa gatttattac cctcatacat cactagaata 5641 tgataatgct cttttttcat cctatcttct gtatcagtat ccctatcatg taatggagac 5701 actacaaatt gaatgtgtaa ctcttttaaa tactctaacc actcggcttt tgctgattct 5761 ggatataaaa caaatgtcca attacgtcct cttgaatttt tcttgttttc agtttctttt 5821 attacatttt cgctcatgat ataataacgg tgctaataca tttaacaaaa tttagtcata 5881 gataggcagc atgccagtgc tgtctatctt tttttgttta aaatgcaccg tattcctcct 5941 ttgcatattt ttttattaga ataccggttg catctgattt gctaatatta tatttttctt 6001 tgattctatt taatatctca ttttcttctg ttgtaagtct taaagtaaca gcaacttttt 6061 tctcttcttt tctatctaca accatcactg tacctcccaa catctgtttt tttcacttta 6121 acataaaaaa caacctttta acattaaaaa cccaatattt atttatttgt ttggacaatg 6181 gacaatggac acctaggggg gaggtcgtag taccccccta tgttttctcc cctaaataac 6241 cccaaaaatc taagaaaaaa agacctcaaa aaggtcttta attaacatct caaatttcgc 6301 atttattcca atttcctttt tgcgtgtgat gcgctgcgtc cattaaaaat cctagagctt 6361 tgcaaccgaa agttaatagc tgtcgctact actttcgctt acgctctaag tatattttaa 6421 ggactgtcac acgcaaaaag ttttctcggc ataaaagtac ctctacatct ctaaatcgtc 6481 tgtacgctgt ttctcacgct ttctatcgac cttctggaca ttatcctgta caacatccat 6541 aaactgtccc acacgctcga atttggaatc attaaagaat ttctctttaa gcctattaaa 6601 ccctttctca aacccaggga aattcgccct cgcagcacga tataaagtca ctgtactatc 6661 ttgaaatttc tctgatacat tcaactgctc attcaaacta tcattctctc gctttaattt 6721 attaacctct ttactttttt cgtgataccc ctctttccat gtattcacta cttctttcaa 6781 actctctcta cgttttttta attcttgatt ttctgtgtaa tagtctgtgc tcttaatatt 6841 ttcgtaatca tcaacaatcc gttcctgcct cgcgcgtttc ggtgatgacg gtgaaaacct 6901 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag 6961 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca 7021 gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga gcagattgta 7081 ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc 7141 atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 7201 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 7261 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 7321 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 7381 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 7441 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 7501 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 7561 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 7621 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 7681 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 7741 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 7801 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 7861 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 7921 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 7981 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 8041 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc 8101 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga 8161 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca 8221 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc 8281 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat 8341 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc 8401 attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt 8461 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc 8521 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg 8581 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt 8641 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg 8701 gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga 8761 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg 8821 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg 8881 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt 8941 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc 9001 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca 9061 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat 9121 aaaaataggc gtatcacgag gccctttcgt cttcaa //