LOCUS pRFP-F 8746 bp DNA circular 26-JUL-2012 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|632672767| COMMENT VNTDBDATE|632673080| COMMENT LSOWNER| COMMENT VNTNAME|pDSRED-F for NARSA| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS complement(7681..8541) /vntifkey="4" /label=AmpR CDS complement(2009..2659) /vntifkey="4" /label=G+\CmR rep_origin 4759..5924 /vntifkey="33" /label=pE194\ORI /note="HhaI or CfoI fragment of pE194 known to replicate" rep_origin complement(6899..7528) /vntifkey="33" /label=pBR322\ori misc_feature 3165..4494 /vntifkey="21" /label=Counter\selection misc_feature 7..516 /vntifkey="21" /label=TN\up misc_feature 1238..1764 /vntifkey="21" /label=TN\down CDS 554..1231 /vntifkey="4" /label=dsRED misc_feature 526..553 /vntifkey="21" /label=TIR BASE COUNT 2652 a 1715 c 1609 g 2770 t ORIGIN 1 gaattctctc tagacacata gatggcgtcg cggcgcgccg gagatataca tatgagtaaa 61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat 121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc 181 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact 241 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt 301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac 361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc 421 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac 481 aactataact cacacaatgt atacatcatg gcaggctaag ctaggtgatt aactttataa 541 ggaggaaaaa catatggata atacagaaga tgttattaaa gaatttatgc gttttaaagt 601 tcgtatggaa ggttcagtta atggtcatga atttgaaatt gaaggtgaag gtgaaggacg 661 tccatatgaa ggtacacaaa cagcaaaatt aaaagttaca aaaggtggtc cattaccatt 721 tgcatgggat attttatcac cacaatttca atatggttca aaagtttatg ttaaacatcc 781 agcagatatt ccagattata agaaattatc atttcctgaa ggttttaaat gggaacgtgt 841 tatgaatttt gaagatggtg gtgttgttac agttacacaa gattcatcat tacaagatgg 901 ttgttttatc tataaagtta aatttattgg tgttaatttt ccatcagatg gtccagttat 961 gcaaaagaaa acaatgggtt gggaaccatc aacagaacgt ttatatccac gtgatggtgt 1021 tttaaaaggt gaaattcata aagcattgaa attaaaagat ggtggtcatt atttagttga 1081 atttaaatca atttatatgg caaaaaaacc agttcaatta ccaggttatt attatgttga 1141 ttcaaaatta gatattacat cacataatga agattataca attgttgaac aatatgaacg 1201 tacagaaggt cgtcatcatt tgtttttata acctagcgcc ataccacaga tgttccagat 1261 aaatattgga agctatatac gtactttgtt tcaaaatggg tcaatcgaga atatcgtcaa 1321 ctgtttacta aaaatcagtt tcatcaagca atgaaacacg ccaaagtaaa caatttaagt 1381 accgttactt atgagcaagt attgtctatt tttaatagtt atctattatt taacgggagg 1441 aaataattct atgagtcgct tttgtaaatt tggaaagtta cacgttacta aagggaatgt 1501 agataaatta ttaggtatac tactgacagc ttccaaggag ctaaagaggt ccctagcgcc 1561 tacggggaat ttgtatcgat aaggaataga tttaaaaatt tcgctgttat tttgtacaat 1621 aaggataaat ttgaatggta ccataaacga ccgtttatgg taccatttca ttttcctgct 1681 ttttctaaat gttttttaag taaatcaagt accaaaatcc gttccttttt catagttcct 1741 atatagttat acgcgtctag agacgtcgac ctgcaggcat gcaagctctg atttcacttt 1801 ttgcattcta caaactgcat aactcatatg taaatcgctc ctttttaggt ggcacaaatg 1861 tgaggcattt tcgctctttc cggcgaggct agttaccctt aagttattgg tatgactggt 1921 tttaagcgca aaaaaagttg ctttttcgta cctattaatg tatcgttaga aaaccgactg 1981 taaaaagtac agtcggcatt atctcatatt ataaaagcca gtcattaggc ctatctgaca 2041 attcctgaat agagttcata aacaatcctg catgataacc atcacaaaca gaatgatgta 2101 cctgtaaaga tagcggtaaa tatattgaat tacctttatt aatgaatttt cctgctgtaa 2161 taatgggtag aaggtaatta ctattattat tgatatttaa gttaaaccca gtaaatgaag 2221 tccatggaat aatagaaaga gaaaaagcat tttcaggtat aggtgttttg ggaaacaatt 2281 tccccgaacc attatatttc tctacatcag aaaggtataa atcataaaac tctttgaagt 2341 cattctttac aggagtccaa ataccagaga atgttttaga tacaccatca aaaattgtat 2401 aaagtggctc taacttatcc caataaccta actctccgtc gctattgtaa ccagttctaa 2461 aagctgtatt tgagtttatc acccttgtca ctaagaaaat aaatgcaggg taaaatttat 2521 atccttcttg ttttatgttt cggtataaaa cactaatatc aatttctgtg gttatactaa 2581 aagtcgtttg ttggttcaaa taatgattaa atatctcttt tctcttccaa ttgtctaaat 2641 caattttatt aaagttcatt tgatatgcct cctaaatttt tatctaaagt gaatttagga 2701 ggcttacttg tctgctttct tcattagaat caatcctttt ttaaaagtca atattactgt 2761 aacataaata tatattttaa aaatatccca ctttatccaa ttttcgtttg ttgaactaat 2821 gggtgcttta gttgaagaat aaagaccaca ttaaaaaatg tggtcttttg tgttttttta 2881 aaggatttga gcgtagcgaa aaatcctttt ctttcttatc ttgataataa gggtaactat 2941 tgccgggata gactgtaaca ttctcacgca taaaatcccc tttcattttc taatgtaaat 3001 ctattacctt attattaatt caattcgctc ataattaatc ctttttctta ttacgcaaaa 3061 tggcccgatt taagcacacc ctttattccg ttaatgcgcc atgacagcca tgataattac 3121 taatactagg agaagttaat aaatacgggt gatctaatga ttcaatgatt caaacccttg 3181 tgaacttctt tagaacaaaa gaggttcgta acaagatttt cttcacacta gcaatgttag 3241 taatttttaa aatagggact tatataccag ctccaggagt aaatcctgca gcttttgata 3301 atccccaagg ttctcaaggt gccactgagt tattaaatac ttttggtggc ggagccttga 3361 aacgattttc tatttttgca atgggtattg taccctacat cactgcatca atcgtaatgc 3421 aattattaca aatggatatt gtccctaaat tctcagaatg ggcaaaacaa ggtgaagtag 3481 gtagaagaaa gttaaataac gttactcgtt atttagcaat ttctttagca tttatccaat 3541 ctataggtat ggcattccaa tttaataatt atctcaaagg tgcgctgatt atcaatcagt 3601 caattatgag ttatttatta atagcactag ttttgacagc aggaactgct ttcttaatat 3661 ggcttggtga tcaaatcact cagttcggtg ttggtaatgg tatttctatt atcatattcc 3721 catcaagctt attttaatta tactctatca atgatagagt gtcaatattt tttttagttt 3781 ttcatgaact cgatcgaggg gatccaaata aaaaactagt ttgacaaata actctatcaa 3841 tgatagagtg tcaacaaaaa ggaggaatta atgatgtcta gattagataa aagtaaagtg 3901 attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac aacccgtaaa 3961 ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa aaataagcgg 4021 gctttgctcg acgccttagc cattgagatg ttagataggc accatactca cttttgccct 4081 ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt tagatgtgct 4141 ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac agaaaaacag 4201 tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc actagagaat 4261 gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt ggaagatcaa 4321 gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat gccgccatta 4381 ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt cttattcggc 4441 cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg gtcttaaaag 4501 cagcataacc tttttccgtg atggtaactt cacggtaacc aagataacaa agaatacaag 4561 aaaatattta caaaaaatca atttaacaat tccttaaaac atgcaggaat tgacgattta 4621 aacaatatta gctttgaaca attcttatct cttttcaata gctataaatt atttaataag 4681 taagttaagg gatgcataaa ctgcatccct taacttgttt ttcgtgtgcc tattttttgt 4741 gaatcgatta tgtcttttgc gcagtcggct taaaccagtt ttcgctggtg cgaaaaaaga 4801 gtgtcttgtg acacctaaat tcaaaatcta tcggtcagat ttataccgat ttgattttat 4861 atattcttga ataacatacg ccgagttatc acataaaagc gggaaccaat catcaaattt 4921 aaacttcatt gcataatcca ttaaactctt aaattctacg attccttgtt catcaataaa 4981 ctcaatcatt tctttaatta atttatatct atctgttgtt gttttcttta ataattcatc 5041 aacatctaca ccgccataaa ctatcatatc ttctttttga tatttaaatt tattaggatc 5101 gtccatgtga agcatatatc tcacaagacc tttcacactt cctgcaatct gcggaatagt 5161 cgcattcaat tcttctgtaa ttatttttat ctgttcataa gatttattac cctcatacat 5221 cactagaata tgataatgct cttttttcat cctatcttct gtatcagtat ccctatcatg 5281 taatggagac actacaaatt gaatgtgtaa ctcttttaaa tactctaacc actcggcttt 5341 tgctgattct ggatataaaa caaatgtcca attacgtcct cttgaatttt tcttgttttc 5401 agtttctttt attacatttt cgctcatgat ataataacgg tgctaataca tttaacaaaa 5461 tttagtcata gataggcagc atgccagtgc tgtctatctt tttttgttta aaatgcaccg 5521 tattcctcct ttgcatattt ttttattaga ataccggttg catctgattt gctaatatta 5581 tatttttctt tgattctatt taatatctca ttttcttctg ttgtaagtct taaagtaaca 5641 gcaacttttt tctcttcttt tctatctaca accatcactg tacctcccaa catctgtttt 5701 tttcacttta acataaaaaa caacctttta acattaaaaa cccaatattt atttatttgt 5761 ttggacaatg gacaatggac acctaggggg gaggtcgtag taccccccta tgttttctcc 5821 cctaaataac cccaaaaatc taagaaaaaa agacctcaaa aaggtcttta attaacatct 5881 caaatttcgc atttattcca atttcctttt tgcgtgtgat gcgctgcgtc cattaaaaat 5941 cctagagctt tgcaaccgaa agttaatagc tgtcgctact actttcgctt acgctctaag 6001 tatattttaa ggactgtcac acgcaaaaag ttttctcggc ataaaagtac ctctacatct 6061 ctaaatcgtc tgtacgctgt ttctcacgct ttctatcgac cttctggaca ttatcctgta 6121 caacatccat aaactgtccc acacgctcga atttggaatc attaaagaat ttctctttaa 6181 gcctattaaa ccctttctca aacccaggga aattcgccct cgcagcacga tataaagtca 6241 ctgtactatc ttgaaatttc tctgatacat tcaactgctc attcaaacta tcattctctc 6301 gctttaattt attaacctct ttactttttt cgtgataccc ctctttccat gtattcacta 6361 cttctttcaa actctctcta cgttttttta attcttgatt ttctgtgtaa tagtctgtgc 6421 tcttaatatt ttcgtaatca tcaacaatcc gttcctgcct cgcgcgtttc ggtgatgacg 6481 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 6541 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 6601 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 6661 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 6721 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 6781 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 6841 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 6901 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 6961 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 7021 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 7081 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 7141 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 7201 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 7261 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 7321 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 7381 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 7441 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 7501 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 7561 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 7621 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 7681 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 7741 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 7801 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 7861 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 7921 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 7981 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 8041 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 8101 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 8161 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 8221 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 8281 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 8341 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 8401 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 8461 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 8521 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 8581 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 8641 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 8701 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaa //