LOCUS pKAN 9019 bp DNA circular 12-MAR-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|656344783| COMMENT VNTDBDATE|656344783| COMMENT LSOWNER| COMMENT VNTNAME|pKAN for website| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS complement(7954..8814) /vntifkey="4" /label=AmpR CDS complement(2282..2932) /vntifkey="4" /label=G+\CmR misc_marker 5032..6197 /vntifkey="22" /label=pE194\ORI /note="HhaI or CfoI fragment of pE194 known to replicate" rep_origin complement(7172..7801) /vntifkey="33" /label=pBR322\ori misc_feature 3438..4767 /vntifkey="21" /label=counter\selection primer_bind complement(2121..2146) /vntifkey="28" /label=JBCL10R misc_feature 7..516 /vntifkey="21" /label=TN\up misc_feature 1511..2037 /vntifkey="21" /label=TN\down misc_feature 708..1502 /vntifkey="21" /label=aphA-3 BASE COUNT 2698 a 1801 c 1731 g 2789 t ORIGIN 1 gaattctctc tagacacata gatggcgtcg cggcgcgccg gagatataca tatgagtaaa 61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat 121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc 181 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact 241 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt 301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac 361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc 421 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac 481 aactataact cacacaatgt atacatcatg gcaggctaag ctaggggttt caaaatcggc 541 tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg 601 tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct 661 tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga 721 gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag 781 gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa 841 tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc 901 tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct 961 ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg 1021 aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca 1081 tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt 1141 acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat 1201 ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg 1261 tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg 1321 gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg 1381 tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac 1441 tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt 1501 agtacctagc gccataccac agatgttcca gataaatatt ggaagctata tacgtacttt 1561 gtttcaaaat gggtcaatcg agaatatcgt caactgttta ctaaaaatca gtttcatcaa 1621 gcaatgaaac acgccaaagt aaacaattta agtaccgtta cttatgagca agtattgtct 1681 atttttaata gttatctatt atttaacggg aggaaataat tctatgagtc gcttttgtaa 1741 atttggaaag ttacacgtta ctaaagggaa tgtagataaa ttattaggta tactactgac 1801 agcttccaag gagctaaaga ggtccctagc gcctacgggg aatttgtatc gataaggaat 1861 agatttaaaa atttcgctgt tattttgtac aataaggata aatttgaatg gtaccataaa 1921 cgaccgttta tggtaccatt tcattttcct gctttttcta aatgtttttt aagtaaatca 1981 agtaccaaaa tccgttcctt tttcatagtt cctatatagt tatacgcgtc tagagacgtc 2041 gacctgcagg catgcaagct ctgatttcac tttttgcatt ctacaaactg cataactcat 2101 atgtaaatcg ctccttttta ggtggcacaa atgtgaggca ttttcgctct ttccggcgag 2161 gctagttacc cttaagttat tggtatgact ggttttaagc gcaaaaaaag ttgctttttc 2221 gtacctatta atgtatcgtt agaaaaccga ctgtaaaaag tacagtcggc attatctcat 2281 attataaaag ccagtcatta ggcctatctg acaattcctg aatagagttc ataaacaatc 2341 ctgcatgata accatcacaa acagaatgat gtacctgtaa agatagcggt aaatatattg 2401 aattaccttt attaatgaat tttcctgctg taataatggg tagaaggtaa ttactattat 2461 tattgatatt taagttaaac ccagtaaatg aagtccatgg aataatagaa agagaaaaag 2521 cattttcagg tataggtgtt ttgggaaaca atttccccga accattatat ttctctacat 2581 cagaaaggta taaatcataa aactctttga agtcattctt tacaggagtc caaataccag 2641 agaatgtttt agatacacca tcaaaaattg tataaagtgg ctctaactta tcccaataac 2701 ctaactctcc gtcgctattg taaccagttc taaaagctgt atttgagttt atcacccttg 2761 tcactaagaa aataaatgca gggtaaaatt tatatccttc ttgttttatg tttcggtata 2821 aaacactaat atcaatttct gtggttatac taaaagtcgt ttgttggttc aaataatgat 2881 taaatatctc ttttctcttc caattgtcta aatcaatttt attaaagttc atttgatatg 2941 cctcctaaat ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag 3001 aatcaatcct tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc 3061 ccactttatc caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaagacc 3121 acattaaaaa atgtggtctt ttgtgttttt ttaaaggatt tgagcgtagc gaaaaatcct 3181 tttctttctt atcttgataa taagggtaac tattgccggg atagactgta acattctcac 3241 gcataaaatc ccctttcatt ttctaatgta aatctattac cttattatta attcaattcg 3301 ctcataatta atcctttttc ttattacgca aaatggcccg atttaagcac accctttatt 3361 ccgttaatgc gccatgacag ccatgataat tactaatact aggagaagtt aataaatacg 3421 ggtgatctaa tgattcaatg attcaaaccc ttgtgaactt ctttagaaca aaagaggttc 3481 gtaacaagat tttcttcaca ctagcaatgt tagtaatttt taaaataggg acttatatac 3541 cagctccagg agtaaatcct gcagcttttg ataatcccca aggttctcaa ggtgccactg 3601 agttattaaa tacttttggt ggcggagcct tgaaacgatt ttctattttt gcaatgggta 3661 ttgtacccta catcactgca tcaatcgtaa tgcaattatt acaaatggat attgtcccta 3721 aattctcaga atgggcaaaa caaggtgaag taggtagaag aaagttaaat aacgttactc 3781 gttatttagc aatttcttta gcatttatcc aatctatagg tatggcattc caatttaata 3841 attatctcaa aggtgcgctg attatcaatc agtcaattat gagttattta ttaatagcac 3901 tagttttgac agcaggaact gctttcttaa tatggcttgg tgatcaaatc actcagttcg 3961 gtgttggtaa tggtatttct attatcatat tcccatcaag cttattttaa ttatactcta 4021 tcaatgatag agtgtcaata ttttttttag tttttcatga actcgatcga ggggatccaa 4081 ataaaaaact agtttgacaa ataactctat caatgataga gtgtcaacaa aaaggaggaa 4141 ttaatgatgt ctagattaga taaaagtaaa gtgattaaca gcgcattaga gctgcttaat 4201 gaggtcggaa tcgaaggttt aacaacccgt aaactcgccc agaagctagg tgtagagcag 4261 cctacattgt attggcatgt aaaaaataag cgggctttgc tcgacgcctt agccattgag 4321 atgttagata ggcaccatac tcacttttgc cctttagaag gggaaagctg gcaagatttt 4381 ttacgtaata acgctaaaag ttttagatgt gctttactaa gtcatcgcga tggagcaaaa 4441 gtacatttag gtacacggcc tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc 4501 tttttatgcc aacaaggttt ttcactagag aatgcattat atgcactcag cgctgtgggg 4561 cattttactt taggttgcgt attggaagat caagagcatc aagtcgctaa agaagaaagg 4621 gaaacaccta ctactgatag tatgccgcca ttattacgac aagctatcga attatttgat 4681 caccaaggtg cagagccagc cttcttattc ggccttgaat tgatcatatg cggattagaa 4741 aaacaactta aatgtgaaag tgggtcttaa aagcagcata acctttttcc gtgatggtaa 4801 cttcacggta accaagataa caaagaatac aagaaaatat ttacaaaaaa tcaatttaac 4861 aattccttaa aacatgcagg aattgacgat ttaaacaata ttagctttga acaattctta 4921 tctcttttca atagctataa attatttaat aagtaagtta agggatgcat aaactgcatc 4981 ccttaacttg tttttcgtgt gcctattttt tgtgaatcga ttatgtcttt tgcgcagtcg 5041 gcttaaacca gttttcgctg gtgcgaaaaa agagtgtctt gtgacaccta aattcaaaat 5101 ctatcggtca gatttatacc gatttgattt tatatattct tgaataacat acgccgagtt 5161 atcacataaa agcgggaacc aatcatcaaa tttaaacttc attgcataat ccattaaact 5221 cttaaattct acgattcctt gttcatcaat aaactcaatc atttctttaa ttaatttata 5281 tctatctgtt gttgttttct ttaataattc atcaacatct acaccgccat aaactatcat 5341 atcttctttt tgatatttaa atttattagg atcgtccatg tgaagcatat atctcacaag 5401 acctttcaca cttcctgcaa tctgcggaat agtcgcattc aattcttctg taattatttt 5461 tatctgttca taagatttat taccctcata catcactaga atatgataat gctctttttt 5521 catcctatct tctgtatcag tatccctatc atgtaatgga gacactacaa attgaatgtg 5581 taactctttt aaatactcta accactcggc ttttgctgat tctggatata aaacaaatgt 5641 ccaattacgt cctcttgaat ttttcttgtt ttcagtttct tttattacat tttcgctcat 5701 gatataataa cggtgctaat acatttaaca aaatttagtc atagataggc agcatgccag 5761 tgctgtctat ctttttttgt ttaaaatgca ccgtattcct cctttgcata tttttttatt 5821 agaataccgg ttgcatctga tttgctaata ttatattttt ctttgattct atttaatatc 5881 tcattttctt ctgttgtaag tcttaaagta acagcaactt ttttctcttc ttttctatct 5941 acaaccatca ctgtacctcc caacatctgt ttttttcact ttaacataaa aaacaacctt 6001 ttaacattaa aaacccaata tttatttatt tgtttggaca atggacaatg gacacctagg 6061 ggggaggtcg tagtaccccc ctatgttttc tcccctaaat aaccccaaaa atctaagaaa 6121 aaaagacctc aaaaaggtct ttaattaaca tctcaaattt cgcatttatt ccaatttcct 6181 ttttgcgtgt gatgcgctgc gtccattaaa aatcctagag ctttgcaacc gaaagttaat 6241 agctgtcgct actactttcg cttacgctct aagtatattt taaggactgt cacacgcaaa 6301 aagttttctc ggcataaaag tacctctaca tctctaaatc gtctgtacgc tgtttctcac 6361 gctttctatc gaccttctgg acattatcct gtacaacatc cataaactgt cccacacgct 6421 cgaatttgga atcattaaag aatttctctt taagcctatt aaaccctttc tcaaacccag 6481 ggaaattcgc cctcgcagca cgatataaag tcactgtact atcttgaaat ttctctgata 6541 cattcaactg ctcattcaaa ctatcattct ctcgctttaa tttattaacc tctttacttt 6601 tttcgtgata cccctctttc catgtattca ctacttcttt caaactctct ctacgttttt 6661 ttaattcttg attttctgtg taatagtctg tgctcttaat attttcgtaa tcatcaacaa 6721 tccgttcctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 6781 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 6841 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 6901 gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 6961 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc 7021 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 7081 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 7141 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 7201 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 7261 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 7321 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 7381 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 7441 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 7501 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 7561 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 7621 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 7681 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 7741 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 7801 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 7861 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 7921 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 7981 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 8041 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 8101 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 8161 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 8221 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg caggcatcgt 8281 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 8341 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 8401 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 8461 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 8521 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa 8581 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 8641 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 8701 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 8761 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 8821 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 8881 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 8941 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 9001 gaggcccttt cgtcttcaa //