LOCUS pJB38 7015 bp DNA circular 12-MAR-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|656345747| COMMENT VNTDBDATE|656345747| COMMENT LSOWNER| COMMENT VNTNAME|pJB38 for website| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS complement(5950..6810) /vntifkey="4" /label=AmpR CDS complement(278..928) /vntifkey="4" /label=G+\CmR misc_marker 3028..4193 /vntifkey="22" /label=pE194\ORI /note="HhaI or CfoI fragment of pE194 known to replicate" rep_origin complement(5168..5797) /vntifkey="33" /label=pBR322\ori misc_feature 1434..2763 /vntifkey="21" /label=counter\selection BASE COUNT 2054 a 1467 c 1278 g 2216 t ORIGIN 1 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagctctga 61 tttcactttt tgcattctac aaactgcata actcatatgt aaatcgctcc tttttaggtg 121 gcacaaatgt gaggcatttt cgctctttcc ggcgaggcta gttaccctta agttattggt 181 atgactggtt ttaagcgcaa aaaaagttgc tttttcgtac ctattaatgt atcgttagaa 241 aaccgactgt aaaaagtaca gtcggcatta tctcatatta taaaagccag tcattaggcc 301 tatctgacaa ttcctgaata gagttcataa acaatcctgc atgataacca tcacaaacag 361 aatgatgtac ctgtaaagat agcggtaaat atattgaatt acctttatta atgaattttc 421 ctgctgtaat aatgggtaga aggtaattac tattattatt gatatttaag ttaaacccag 481 taaatgaagt ccatggaata atagaaagag aaaaagcatt ttcaggtata ggtgttttgg 541 gaaacaattt ccccgaacca ttatatttct ctacatcaga aaggtataaa tcataaaact 601 ctttgaagtc attctttaca ggagtccaaa taccagagaa tgttttagat acaccatcaa 661 aaattgtata aagtggctct aacttatccc aataacctaa ctctccgtcg ctattgtaac 721 cagttctaaa agctgtattt gagtttatca cccttgtcac taagaaaata aatgcagggt 781 aaaatttata tccttcttgt tttatgtttc ggtataaaac actaatatca atttctgtgg 841 ttatactaaa agtcgtttgt tggttcaaat aatgattaaa tatctctttt ctcttccaat 901 tgtctaaatc aattttatta aagttcattt gatatgcctc ctaaattttt atctaaagtg 961 aatttaggag gcttacttgt ctgctttctt cattagaatc aatccttttt taaaagtcaa 1021 tattactgta acataaatat atattttaaa aatatcccac tttatccaat tttcgtttgt 1081 tgaactaatg ggtgctttag ttgaagaata aagaccacat taaaaaatgt ggtcttttgt 1141 gtttttttaa aggatttgag cgtagcgaaa aatccttttc tttcttatct tgataataag 1201 ggtaactatt gccgggatag actgtaacat tctcacgcat aaaatcccct ttcattttct 1261 aatgtaaatc tattacctta ttattaattc aattcgctca taattaatcc tttttcttat 1321 tacgcaaaat ggcccgattt aagcacaccc tttattccgt taatgcgcca tgacagccat 1381 gataattact aatactagga gaagttaata aatacgggtg atctaatgat tcaatgattc 1441 aaacccttgt gaacttcttt agaacaaaag aggttcgtaa caagattttc ttcacactag 1501 caatgttagt aatttttaaa atagggactt atataccagc tccaggagta aatcctgcag 1561 cttttgataa tccccaaggt tctcaaggtg ccactgagtt attaaatact tttggtggcg 1621 gagccttgaa acgattttct atttttgcaa tgggtattgt accctacatc actgcatcaa 1681 tcgtaatgca attattacaa atggatattg tccctaaatt ctcagaatgg gcaaaacaag 1741 gtgaagtagg tagaagaaag ttaaataacg ttactcgtta tttagcaatt tctttagcat 1801 ttatccaatc tataggtatg gcattccaat ttaataatta tctcaaaggt gcgctgatta 1861 tcaatcagtc aattatgagt tatttattaa tagcactagt tttgacagca ggaactgctt 1921 tcttaatatg gcttggtgat caaatcactc agttcggtgt tggtaatggt atttctatta 1981 tcatattccc atcaagctta ttttaattat actctatcaa tgatagagtg tcaatatttt 2041 ttttagtttt tcatgaactc gatcgagggg atccaaataa aaaactagtt tgacaaataa 2101 ctctatcaat gatagagtgt caacaaaaag gaggaattaa tgatgtctag attagataaa 2161 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca 2221 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa 2281 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac 2341 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt 2401 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca 2461 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca 2521 ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg 2581 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg 2641 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc 2701 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg 2761 tcttaaaagc agcataacct ttttccgtga tggtaacttc acggtaacca agataacaaa 2821 gaatacaaga aaatatttac aaaaaatcaa tttaacaatt ccttaaaaca tgcaggaatt 2881 gacgatttaa acaatattag ctttgaacaa ttcttatctc ttttcaatag ctataaatta 2941 tttaataagt aagttaaggg atgcataaac tgcatccctt aacttgtttt tcgtgtgcct 3001 attttttgtg aatcgattat gtcttttgcg cagtcggctt aaaccagttt tcgctggtgc 3061 gaaaaaagag tgtcttgtga cacctaaatt caaaatctat cggtcagatt tataccgatt 3121 tgattttata tattcttgaa taacatacgc cgagttatca cataaaagcg ggaaccaatc 3181 atcaaattta aacttcattg cataatccat taaactctta aattctacga ttccttgttc 3241 atcaataaac tcaatcattt ctttaattaa tttatatcta tctgttgttg ttttctttaa 3301 taattcatca acatctacac cgccataaac tatcatatct tctttttgat atttaaattt 3361 attaggatcg tccatgtgaa gcatatatct cacaagacct ttcacacttc ctgcaatctg 3421 cggaatagtc gcattcaatt cttctgtaat tatttttatc tgttcataag atttattacc 3481 ctcatacatc actagaatat gataatgctc ttttttcatc ctatcttctg tatcagtatc 3541 cctatcatgt aatggagaca ctacaaattg aatgtgtaac tcttttaaat actctaacca 3601 ctcggctttt gctgattctg gatataaaac aaatgtccaa ttacgtcctc ttgaattttt 3661 cttgttttca gtttctttta ttacattttc gctcatgata taataacggt gctaatacat 3721 ttaacaaaat ttagtcatag ataggcagca tgccagtgct gtctatcttt ttttgtttaa 3781 aatgcaccgt attcctcctt tgcatatttt tttattagaa taccggttgc atctgatttg 3841 ctaatattat atttttcttt gattctattt aatatctcat tttcttctgt tgtaagtctt 3901 aaagtaacag caactttttt ctcttctttt ctatctacaa ccatcactgt acctcccaac 3961 atctgttttt ttcactttaa cataaaaaac aaccttttaa cattaaaaac ccaatattta 4021 tttatttgtt tggacaatgg acaatggaca cctagggggg aggtcgtagt acccccctat 4081 gttttctccc ctaaataacc ccaaaaatct aagaaaaaaa gacctcaaaa aggtctttaa 4141 ttaacatctc aaatttcgca tttattccaa tttccttttt gcgtgtgatg cgctgcgtcc 4201 attaaaaatc ctagagcttt gcaaccgaaa gttaatagct gtcgctacta ctttcgctta 4261 cgctctaagt atattttaag gactgtcaca cgcaaaaagt tttctcggca taaaagtacc 4321 tctacatctc taaatcgtct gtacgctgtt tctcacgctt tctatcgacc ttctggacat 4381 tatcctgtac aacatccata aactgtccca cacgctcgaa tttggaatca ttaaagaatt 4441 tctctttaag cctattaaac cctttctcaa acccagggaa attcgccctc gcagcacgat 4501 ataaagtcac tgtactatct tgaaatttct ctgatacatt caactgctca ttcaaactat 4561 cattctctcg ctttaattta ttaacctctt tacttttttc gtgatacccc tctttccatg 4621 tattcactac ttctttcaaa ctctctctac gtttttttaa ttcttgattt tctgtgtaat 4681 agtctgtgct cttaatattt tcgtaatcat caacaatccg ttcctgcctc gcgcgtttcg 4741 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 4801 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 4861 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc 4921 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg 4981 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg 5041 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 5101 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 5161 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 5221 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 5281 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 5341 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 5401 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 5461 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 5521 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 5581 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt 5641 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 5701 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 5761 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 5821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 5881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg 5941 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 6001 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 6061 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc 6121 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 6181 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 6241 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat 6301 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg 6361 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 6421 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 6481 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 6541 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcgccacata gcagaacttt 6601 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 6661 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac 6721 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 6781 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 6841 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 6901 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 6961 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcaa //