LOCUS pGFP-R 8791 bp DNA circular 12-MAR-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|656345988| COMMENT VNTDBDATE|656345988| COMMENT LSOWNER| COMMENT VNTNAME|pGFP-R for website| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS complement(7726..8586) /vntifkey="4" /label=AmpR CDS complement(2054..2704) /vntifkey="4" /label=G+\CmR misc_marker 4804..5969 /vntifkey="22" /label=pE194\ORI /note="HhaI or CfoI fragment of pE194 known to replicate" rep_origin complement(6944..7573) /vntifkey="33" /label=pBR322\ori misc_feature 3210..4539 /vntifkey="21" /label=counter\selection misc_feature 7..516 /vntifkey="21" /label=TN\up misc_feature 1283..1809 /vntifkey="21" /label=TN\down CDS complement(526..1248) /vntifkey="4" /label=sGFP enhancer complement(1249..1276) /vntifkey="9" /label=TIR BASE COUNT 2596 a 1790 c 1635 g 2770 t ORIGIN 1 gaattctctc tagacacata gatggcgtcg cggcgcgccg gagatataca tatgagtaaa 61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat 121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc 181 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact 241 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt 301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac 361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc 421 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac 481 aactataact cacacaatgt atacatcatg gcaggctaag ctaggttatt tgtagagctc 541 atccatgcca tgtgtaatcc cagcagcagt tacaaactca agaaggacca tgtggtcacg 601 cttttcgtta ggatctttcg aaaggacaga ttgtgtcgac aggtaatggt tgtctggtaa 661 aaggacaggg ccatcgccaa ttggagtatt ttgttgataa tggtctgcta gttgaacgga 721 accatcttca acgttgtggc gaattttgaa gttagctttg attccattct tttgtttgtc 781 tgccgtgatg tatacattgt gtgagttaaa gttgtactcg agtttgtgtc caagaatgtt 841 tccatcttct ttaaaatcaa taccctttaa ctcgatacga ttaacaaggg tatcaccttc 901 aaacttgact tcagcacgcg tcttgtaggt cccgtcatct ttgaaagata tagtgcgttc 961 ctgtacataa ccttcaggca tggcactctt gaaaaagtca tgacgtttca tgtgatccgg 1021 ataacgggaa aagcattgaa caccataggt cagagtagtg acaagtgttg gccacggaac 1081 aggtagtttt ccagtagtgc aaataaattt aagggtgagt tttccgtttg tagcatcacc 1141 ttcaccctct ccacggacag aaaatttgtg cccattaaca tcaccatcta attcaacaag 1201 aattgggaca actccagtga aaagttcttc tcctttgctc ccgggcatat gtttttcctc 1261 cttataaagt taatcaccta gcgccatacc acagatgttc cagataaata ttggaagcta 1321 tatacgtact ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat 1381 cagtttcatc aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt tacttatgag 1441 caagtattgt ctatttttaa tagttatcta ttatttaacg ggaggaaata attctatgag 1501 tcgcttttgt aaatttggaa agttacacgt tactaaaggg aatgtagata aattattagg 1561 tatactactg acagcttcca aggagctaaa gaggtcccta gcgcctacgg ggaatttgta 1621 tcgataagga atagatttaa aaatttcgct gttattttgt acaataagga taaatttgaa 1681 tggtaccata aacgaccgtt tatggtacca tttcattttc ctgctttttc taaatgtttt 1741 ttaagtaaat caagtaccaa aatccgttcc tttttcatag ttcctatata gttatacgcg 1801 tctagagacg tcgacctgca ggcatgcaag ctctgatttc actttttgca ttctacaaac 1861 tgcataactc atatgtaaat cgctcctttt taggtggcac aaatgtgagg cattttcgct 1921 ctttccggcg aggctagtta cccttaagtt attggtatga ctggttttaa gcgcaaaaaa 1981 agttgctttt tcgtacctat taatgtatcg ttagaaaacc gactgtaaaa agtacagtcg 2041 gcattatctc atattataaa agccagtcat taggcctatc tgacaattcc tgaatagagt 2101 tcataaacaa tcctgcatga taaccatcac aaacagaatg atgtacctgt aaagatagcg 2161 gtaaatatat tgaattacct ttattaatga attttcctgc tgtaataatg ggtagaaggt 2221 aattactatt attattgata tttaagttaa acccagtaaa tgaagtccat ggaataatag 2281 aaagagaaaa agcattttca ggtataggtg ttttgggaaa caatttcccc gaaccattat 2341 atttctctac atcagaaagg tataaatcat aaaactcttt gaagtcattc tttacaggag 2401 tccaaatacc agagaatgtt ttagatacac catcaaaaat tgtataaagt ggctctaact 2461 tatcccaata acctaactct ccgtcgctat tgtaaccagt tctaaaagct gtatttgagt 2521 ttatcaccct tgtcactaag aaaataaatg cagggtaaaa tttatatcct tcttgtttta 2581 tgtttcggta taaaacacta atatcaattt ctgtggttat actaaaagtc gtttgttggt 2641 tcaaataatg attaaatatc tcttttctct tccaattgtc taaatcaatt ttattaaagt 2701 tcatttgata tgcctcctaa atttttatct aaagtgaatt taggaggctt acttgtctgc 2761 tttcttcatt agaatcaatc cttttttaaa agtcaatatt actgtaacat aaatatatat 2821 tttaaaaata tcccacttta tccaattttc gtttgttgaa ctaatgggtg ctttagttga 2881 agaataaaga ccacattaaa aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta 2941 gcgaaaaatc cttttctttc ttatcttgat aataagggta actattgccg ggatagactg 3001 taacattctc acgcataaaa tcccctttca ttttctaatg taaatctatt accttattat 3061 taattcaatt cgctcataat taatcctttt tcttattacg caaaatggcc cgatttaagc 3121 acacccttta ttccgttaat gcgccatgac agccatgata attactaata ctaggagaag 3181 ttaataaata cgggtgatct aatgattcaa tgattcaaac ccttgtgaac ttctttagaa 3241 caaaagaggt tcgtaacaag attttcttca cactagcaat gttagtaatt tttaaaatag 3301 ggacttatat accagctcca ggagtaaatc ctgcagcttt tgataatccc caaggttctc 3361 aaggtgccac tgagttatta aatacttttg gtggcggagc cttgaaacga ttttctattt 3421 ttgcaatggg tattgtaccc tacatcactg catcaatcgt aatgcaatta ttacaaatgg 3481 atattgtccc taaattctca gaatgggcaa aacaaggtga agtaggtaga agaaagttaa 3541 ataacgttac tcgttattta gcaatttctt tagcatttat ccaatctata ggtatggcat 3601 tccaatttaa taattatctc aaaggtgcgc tgattatcaa tcagtcaatt atgagttatt 3661 tattaatagc actagttttg acagcaggaa ctgctttctt aatatggctt ggtgatcaaa 3721 tcactcagtt cggtgttggt aatggtattt ctattatcat attcccatca agcttatttt 3781 aattatactc tatcaatgat agagtgtcaa tatttttttt agtttttcat gaactcgatc 3841 gaggggatcc aaataaaaaa ctagtttgac aaataactct atcaatgata gagtgtcaac 3901 aaaaaggagg aattaatgat gtctagatta gataaaagta aagtgattaa cagcgcatta 3961 gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta 4021 ggtgtagagc agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc 4081 ttagccattg agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc 4141 tggcaagatt ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc 4201 gatggagcaa aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa 4261 aatcaattag cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc 4321 agcgctgtgg ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct 4381 aaagaagaaa gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc 4441 gaattatttg atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata 4501 tgcggattag aaaaacaact taaatgtgaa agtgggtctt aaaagcagca taaccttttt 4561 ccgtgatggt aacttcacgg taaccaagat aacaaagaat acaagaaaat atttacaaaa 4621 aatcaattta acaattcctt aaaacatgca ggaattgacg atttaaacaa tattagcttt 4681 gaacaattct tatctctttt caatagctat aaattattta ataagtaagt taagggatgc 4741 ataaactgca tcccttaact tgtttttcgt gtgcctattt tttgtgaatc gattatgtct 4801 tttgcgcagt cggcttaaac cagttttcgc tggtgcgaaa aaagagtgtc ttgtgacacc 4861 taaattcaaa atctatcggt cagatttata ccgatttgat tttatatatt cttgaataac 4921 atacgccgag ttatcacata aaagcgggaa ccaatcatca aatttaaact tcattgcata 4981 atccattaaa ctcttaaatt ctacgattcc ttgttcatca ataaactcaa tcatttcttt 5041 aattaattta tatctatctg ttgttgtttt ctttaataat tcatcaacat ctacaccgcc 5101 ataaactatc atatcttctt tttgatattt aaatttatta ggatcgtcca tgtgaagcat 5161 atatctcaca agacctttca cacttcctgc aatctgcgga atagtcgcat tcaattcttc 5221 tgtaattatt tttatctgtt cataagattt attaccctca tacatcacta gaatatgata 5281 atgctctttt ttcatcctat cttctgtatc agtatcccta tcatgtaatg gagacactac 5341 aaattgaatg tgtaactctt ttaaatactc taaccactcg gcttttgctg attctggata 5401 taaaacaaat gtccaattac gtcctcttga atttttcttg ttttcagttt cttttattac 5461 attttcgctc atgatataat aacggtgcta atacatttaa caaaatttag tcatagatag 5521 gcagcatgcc agtgctgtct atcttttttt gtttaaaatg caccgtattc ctcctttgca 5581 tattttttta ttagaatacc ggttgcatct gatttgctaa tattatattt ttctttgatt 5641 ctatttaata tctcattttc ttctgttgta agtcttaaag taacagcaac ttttttctct 5701 tcttttctat ctacaaccat cactgtacct cccaacatct gtttttttca ctttaacata 5761 aaaaacaacc ttttaacatt aaaaacccaa tatttattta tttgtttgga caatggacaa 5821 tggacaccta ggggggaggt cgtagtaccc ccctatgttt tctcccctaa ataaccccaa 5881 aaatctaaga aaaaaagacc tcaaaaaggt ctttaattaa catctcaaat ttcgcattta 5941 ttccaatttc ctttttgcgt gtgatgcgct gcgtccatta aaaatcctag agctttgcaa 6001 ccgaaagtta atagctgtcg ctactacttt cgcttacgct ctaagtatat tttaaggact 6061 gtcacacgca aaaagttttc tcggcataaa agtacctcta catctctaaa tcgtctgtac 6121 gctgtttctc acgctttcta tcgaccttct ggacattatc ctgtacaaca tccataaact 6181 gtcccacacg ctcgaatttg gaatcattaa agaatttctc tttaagccta ttaaaccctt 6241 tctcaaaccc agggaaattc gccctcgcag cacgatataa agtcactgta ctatcttgaa 6301 atttctctga tacattcaac tgctcattca aactatcatt ctctcgcttt aatttattaa 6361 cctctttact tttttcgtga tacccctctt tccatgtatt cactacttct ttcaaactct 6421 ctctacgttt ttttaattct tgattttctg tgtaatagtc tgtgctctta atattttcgt 6481 aatcatcaac aatccgttcc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 6541 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 6601 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 6661 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 6721 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 6781 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 6841 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 6901 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 6961 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 7021 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 7081 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 7141 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 7201 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 7261 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 7321 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 7381 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 7441 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 7501 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 7561 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 7621 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 7681 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 7741 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 7801 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 7861 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 7921 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 7981 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 8041 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 8101 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 8161 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 8221 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 8281 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 8341 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 8401 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 8461 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 8521 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 8581 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 8641 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 8701 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 8761 taggcgtatc acgaggccct ttcgtcttca a //