LOCUS pFP650-R 8773 bp DNA circular 12-MAR-2013 SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|656351548| COMMENT VNTDBDATE|656351548| COMMENT LSOWNER| COMMENT VNTNAME|pFP650-R for website| COMMENT VNTAUTHORNAME|Demo User| FEATURES Location/Qualifiers CDS complement(7708..8568) /vntifkey="4" /label=AmpR CDS complement(2036..2686) /vntifkey="4" /label=G+\CmR misc_marker 4786..5951 /vntifkey="22" /label=pE194\ORI /note="HhaI or CfoI fragment of pE194 known to replicate" rep_origin complement(6926..7555) /vntifkey="33" /label=pBR322\ori misc_feature 3192..4521 /vntifkey="21" /label=counter\selection misc_feature 7..516 /vntifkey="21" /label=TN\up misc_feature 1265..1791 /vntifkey="21" /label=TN\down misc_feature complement(1231..1258) /vntifkey="21" /label=TIR CDS complement(526..1230) /vntifkey="4" /label=eqFP650 BASE COUNT 2641 a 1770 c 1560 g 2802 t ORIGIN 1 gaattctctc tagacacata gatggcgtcg cggcgcgccg gagatataca tatgagtaaa 61 ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat 121 gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg aaaacttacc 181 cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact 241 ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca gcatgacttt 301 ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt caaagatgac 361 gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt taatagaatc 421 gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa attggaatac 481 aactataact cacacaatgt atacatcatg gcaggctaag ctaggttaac tatgacctaa 541 ttttgatggt aaatcacaat aacgagctac agccatttca tgttgttcaa cataagtttc 601 tttatcagct tctttaattc tttctaattt acgatcaaca aaataaaaac caggcatttt 661 taaatttttt gctggttttt tagatctata agttgttttt aaactacaat gtaaataacc 721 accaccaact aattttaaag ccatttgtga atgaccacgt aaaccagaat cagctggata 781 taacatttca gtacttgctt cccaacctaa tgttttcttt tgcattacag gaccatttga 841 tggaaaattt acaccattaa ttttaacatt ataaattaaa caaccatttt gtaaacttgt 901 atcttgagtt gctgttaaaa caccaccatc ttcataagtt gtaatacgtt cccatgtaaa 961 accttcagga aaagattgtt taaagaaatc tggaatacct tgagtatgat taataaatgt 1021 tttactacca tacataaatg atgtagctaa aatatcaaat gcaaaaggta atggaccacc 1081 ttctacaact ttaatttttg cagtttgtgt accttcataa ggtttacctt caccttctga 1141 agtacattta aaatgatgac catttacagt accttccata tataatttca tgtgcatatt 1201 ttcactaatt aattcactat cttcacccat atgtttttcc tccttataaa gttaatcacc 1261 tagcgccata ccacagatgt tccagataaa tattggaagc tatatacgta ctttgtttca 1321 aaatgggtca atcgagaata tcgtcaactg tttactaaaa atcagtttca tcaagcaatg 1381 aaacacgcca aagtaaacaa tttaagtacc gttacttatg agcaagtatt gtctattttt 1441 aatagttatc tattatttaa cgggaggaaa taattctatg agtcgctttt gtaaatttgg 1501 aaagttacac gttactaaag ggaatgtaga taaattatta ggtatactac tgacagcttc 1561 caaggagcta aagaggtccc tagcgcctac ggggaatttg tatcgataag gaatagattt 1621 aaaaatttcg ctgttatttt gtacaataag gataaatttg aatggtacca taaacgaccg 1681 tttatggtac catttcattt tcctgctttt tctaaatgtt ttttaagtaa atcaagtacc 1741 aaaatccgtt cctttttcat agttcctata tagttatacg cgtctagaga cgtcgacctg 1801 caggcatgca agctctgatt tcactttttg cattctacaa actgcataac tcatatgtaa 1861 atcgctcctt tttaggtggc acaaatgtga ggcattttcg ctctttccgg cgaggctagt 1921 tacccttaag ttattggtat gactggtttt aagcgcaaaa aaagttgctt tttcgtacct 1981 attaatgtat cgttagaaaa ccgactgtaa aaagtacagt cggcattatc tcatattata 2041 aaagccagtc attaggccta tctgacaatt cctgaataga gttcataaac aatcctgcat 2101 gataaccatc acaaacagaa tgatgtacct gtaaagatag cggtaaatat attgaattac 2161 ctttattaat gaattttcct gctgtaataa tgggtagaag gtaattacta ttattattga 2221 tatttaagtt aaacccagta aatgaagtcc atggaataat agaaagagaa aaagcatttt 2281 caggtatagg tgttttggga aacaatttcc ccgaaccatt atatttctct acatcagaaa 2341 ggtataaatc ataaaactct ttgaagtcat tctttacagg agtccaaata ccagagaatg 2401 ttttagatac accatcaaaa attgtataaa gtggctctaa cttatcccaa taacctaact 2461 ctccgtcgct attgtaacca gttctaaaag ctgtatttga gtttatcacc cttgtcacta 2521 agaaaataaa tgcagggtaa aatttatatc cttcttgttt tatgtttcgg tataaaacac 2581 taatatcaat ttctgtggtt atactaaaag tcgtttgttg gttcaaataa tgattaaata 2641 tctcttttct cttccaattg tctaaatcaa ttttattaaa gttcatttga tatgcctcct 2701 aaatttttat ctaaagtgaa tttaggaggc ttacttgtct gctttcttca ttagaatcaa 2761 tcctttttta aaagtcaata ttactgtaac ataaatatat attttaaaaa tatcccactt 2821 tatccaattt tcgtttgttg aactaatggg tgctttagtt gaagaataaa gaccacatta 2881 aaaaatgtgg tcttttgtgt ttttttaaag gatttgagcg tagcgaaaaa tccttttctt 2941 tcttatcttg ataataaggg taactattgc cgggatagac tgtaacattc tcacgcataa 3001 aatccccttt cattttctaa tgtaaatcta ttaccttatt attaattcaa ttcgctcata 3061 attaatcctt tttcttatta cgcaaaatgg cccgatttaa gcacaccctt tattccgtta 3121 atgcgccatg acagccatga taattactaa tactaggaga agttaataaa tacgggtgat 3181 ctaatgattc aatgattcaa acccttgtga acttctttag aacaaaagag gttcgtaaca 3241 agattttctt cacactagca atgttagtaa tttttaaaat agggacttat ataccagctc 3301 caggagtaaa tcctgcagct tttgataatc cccaaggttc tcaaggtgcc actgagttat 3361 taaatacttt tggtggcgga gccttgaaac gattttctat ttttgcaatg ggtattgtac 3421 cctacatcac tgcatcaatc gtaatgcaat tattacaaat ggatattgtc cctaaattct 3481 cagaatgggc aaaacaaggt gaagtaggta gaagaaagtt aaataacgtt actcgttatt 3541 tagcaatttc tttagcattt atccaatcta taggtatggc attccaattt aataattatc 3601 tcaaaggtgc gctgattatc aatcagtcaa ttatgagtta tttattaata gcactagttt 3661 tgacagcagg aactgctttc ttaatatggc ttggtgatca aatcactcag ttcggtgttg 3721 gtaatggtat ttctattatc atattcccat caagcttatt ttaattatac tctatcaatg 3781 atagagtgtc aatatttttt ttagtttttc atgaactcga tcgaggggat ccaaataaaa 3841 aactagtttg acaaataact ctatcaatga tagagtgtca acaaaaagga ggaattaatg 3901 atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc 3961 ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca 4021 ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta 4081 gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt 4141 aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat 4201 ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta 4261 tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt 4321 actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca 4381 cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa 4441 ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa 4501 cttaaatgtg aaagtgggtc ttaaaagcag cataaccttt ttccgtgatg gtaacttcac 4561 ggtaaccaag ataacaaaga atacaagaaa atatttacaa aaaatcaatt taacaattcc 4621 ttaaaacatg caggaattga cgatttaaac aatattagct ttgaacaatt cttatctctt 4681 ttcaatagct ataaattatt taataagtaa gttaagggat gcataaactg catcccttaa 4741 cttgtttttc gtgtgcctat tttttgtgaa tcgattatgt cttttgcgca gtcggcttaa 4801 accagttttc gctggtgcga aaaaagagtg tcttgtgaca cctaaattca aaatctatcg 4861 gtcagattta taccgatttg attttatata ttcttgaata acatacgccg agttatcaca 4921 taaaagcggg aaccaatcat caaatttaaa cttcattgca taatccatta aactcttaaa 4981 ttctacgatt ccttgttcat caataaactc aatcatttct ttaattaatt tatatctatc 5041 tgttgttgtt ttctttaata attcatcaac atctacaccg ccataaacta tcatatcttc 5101 tttttgatat ttaaatttat taggatcgtc catgtgaagc atatatctca caagaccttt 5161 cacacttcct gcaatctgcg gaatagtcgc attcaattct tctgtaatta tttttatctg 5221 ttcataagat ttattaccct catacatcac tagaatatga taatgctctt ttttcatcct 5281 atcttctgta tcagtatccc tatcatgtaa tggagacact acaaattgaa tgtgtaactc 5341 ttttaaatac tctaaccact cggcttttgc tgattctgga tataaaacaa atgtccaatt 5401 acgtcctctt gaatttttct tgttttcagt ttcttttatt acattttcgc tcatgatata 5461 ataacggtgc taatacattt aacaaaattt agtcatagat aggcagcatg ccagtgctgt 5521 ctatcttttt ttgtttaaaa tgcaccgtat tcctcctttg catatttttt tattagaata 5581 ccggttgcat ctgatttgct aatattatat ttttctttga ttctatttaa tatctcattt 5641 tcttctgttg taagtcttaa agtaacagca acttttttct cttcttttct atctacaacc 5701 atcactgtac ctcccaacat ctgttttttt cactttaaca taaaaaacaa ccttttaaca 5761 ttaaaaaccc aatatttatt tatttgtttg gacaatggac aatggacacc taggggggag 5821 gtcgtagtac ccccctatgt tttctcccct aaataacccc aaaaatctaa gaaaaaaaga 5881 cctcaaaaag gtctttaatt aacatctcaa atttcgcatt tattccaatt tcctttttgc 5941 gtgtgatgcg ctgcgtccat taaaaatcct agagctttgc aaccgaaagt taatagctgt 6001 cgctactact ttcgcttacg ctctaagtat attttaagga ctgtcacacg caaaaagttt 6061 tctcggcata aaagtacctc tacatctcta aatcgtctgt acgctgtttc tcacgctttc 6121 tatcgacctt ctggacatta tcctgtacaa catccataaa ctgtcccaca cgctcgaatt 6181 tggaatcatt aaagaatttc tctttaagcc tattaaaccc tttctcaaac ccagggaaat 6241 tcgccctcgc agcacgatat aaagtcactg tactatcttg aaatttctct gatacattca 6301 actgctcatt caaactatca ttctctcgct ttaatttatt aacctcttta cttttttcgt 6361 gatacccctc tttccatgta ttcactactt ctttcaaact ctctctacgt ttttttaatt 6421 cttgattttc tgtgtaatag tctgtgctct taatattttc gtaatcatca acaatccgtt 6481 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 6541 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 6601 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt 6661 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 6721 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc 6781 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 6841 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 6901 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 6961 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 7021 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 7081 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 7141 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 7201 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 7261 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 7321 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 7381 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 7441 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 7501 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 7561 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 7621 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 7681 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 7741 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 7801 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 7861 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 7921 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 7981 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc 8041 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 8101 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 8161 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 8221 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 8281 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg ataataccgc 8341 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 8401 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 8461 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 8521 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 8581 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 8641 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 8701 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 8761 ctttcgtctt caa //